Coding

Part:BBa_K1758390:Design

Designed by: Team Bielefeld-CeBiTec 2015   Group: iGEM15_Bielefeld-CeBiTec   (2015-08-22)


His-tagged E. coli tryptophanase under control of the T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1209
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 638
    Illegal AgeI site found at 1259
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was obtained as linear gene synthesis and cloned into pSB1C3 via Gibson Assembly.


Source

This part was obtained by gene synthesis.

References